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. 2011 Oct 17;14(2):294–300. doi: 10.1038/aja.2011.94

Figure 1.

Figure 1

The gene expression of AR in TM4 cells and four stably transfected TM4/AR cell lines. (a) The relative transcriptional activity of AR detected by qRT-PCR. **P<0.01 compared with TM4. (b) The relative expression of AR protein in TM4 and four TM4/AR cell lines detected by Western blot. GAPDH was used as a loading control. AR, androgen receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; qRT-PCR, quantitative reverse-transcription PCR analysis; TM4-1, TM4/AR1 cells; TM4-2, TM4/AR1 cells with AR-cDNA transient transfected; TM4-3, TM4/AR1 cells with AR-cDNA transient transfected and T treatment.