Figure 3.
The effect of transient expression of AR and treatment of T on reporter gene expression controlled by androgen-responsive element in TM4 or TM4/AR cells. The MMTV-Luc luciferase activity in TM4 or TM4/AR cells was induced by cotransfecting AR-cDNA dose dependently in the presence of 100 nmol l−1 T (a) and induced by T in dose-dependent manner when cotransfected with 0.1 µg AR-cDNA (b). Luciferase activity was normalized to β-galactosidase activity and then shown relative to that of no treatment, which was set to 100%. Each value represents the mean±s.d. of experiments performed in triplicate. The data of TM4/AR cells are representative of four TM4/AR cell lines. AR, androgen receptor; MMTV-Luc, mouse mammary tumor virus-luciferase; T, testosterone.