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Mapping of the vhs-dependent endonucleolytic cleavage site in IEX-1 mRNA. (A) Location along the IEX-1 RNA sequence of Ribo1 and Ribo2, two riboprobes antisense to the 3′ UTR, used for Northern blot analysis. (B) HeLa cells were mock-infected or infected with 10 pfu of HSV-1(F) or ΔUL41-mutant virus per cell. Cytoplasmic RNA was purified from cells harvested at the indicated times after mock infection (lanes 1 and 5), infection with HSV-1(F) (lanes 6-10), or ΔUL41 mutant (lanes 11-15).
