TABLE 1 .
SNP differences between the initial (MSHR1043) and 139-month (MSHR6686) B. pseudomallei isolates from P314
| Location in MSHR1043 |
Nucleotide change |
Amino acid change |
Affected gene in MSHR1043 (K96243) |
SNP effecta | Mutated in MSHR1655? |
Affected protein(s) | Putative protein function(s) |
|---|---|---|---|---|---|---|---|
| seq0001 396179 |
C→T | T194M |
D512_01885 (BPSL0128) |
NS; deleterious | No | NtrX | DNA transcription regulator |
| seq0001 684105 |
C→T | A101A |
D512_03260 (BPSL0430) |
S; neutral | No | Thioesterase | Unknown |
| seq0001 798196 |
C→T | A8T |
D512_03780 (BPSL0530) |
NS; neutral | No | HPr kinase, phosphorylase | Cell adhesion, virulence |
| seq0003 154078 |
A→G | H115R |
D512_07998 (BPSL1362) |
NS; neutral | Yes | PstB | Phosphate uptake |
| seq0003 331699 |
T→C | I33V |
D512_08718 (BPSL1981) |
NS; neutral | Yes | GalU | Sugar, LPS metabolism |
| seq0003 397164 |
A→C | L47R |
D512_09003 (BPSL1924) |
NS; deleterious | No | Hypothetical protein | Unknown |
| seq0003 536977 |
G→A | NAe | NA | NA | No | 99 bp upstream of TetR | Unknown |
| seq0003 678249 |
G→T | R2242L |
D512_10148 (BPSL1712) |
NS; neutral | No | Syringomycin synthetase | Phytopathogenesis |
| seq0003 719972 |
T→C | F84L |
D512_10298 (BPSL1689) |
NS; deleterious | No | Hypothetical protein | Unknown |
| seq0003 720544 |
G→A | G85S |
D512_10303 (BPSL1688) |
NS; deleterious | Yes | Hypothetical protein | Unknown |
| seq0003 943033 |
G→A | Q82stop |
D512_11298 (BPSL1505) |
Nonsense; deleterious |
No | RpoS | Universal stress response |
| seq0003 1040609 |
G→A | G263D |
D512_11778 (BPSL1413) |
NS; deleterious | Yes | Glucose-6-phosphate isomerase |
Glycolysis, gluconeogenesis |
| seq0006 91557 |
T→C | L279P |
D512_14441 (BPSL2409) |
NS; deleterious | Yes | ATP binding protein | Molecular transport |
| seq0007 422966 |
G→A | NA | NA | NA | No | NA | Unknown |
| seq0008 15374 |
G→C | I637M |
D512_17625 (BPSL3016) |
NS; deleterious | Yes | Seca | Membrane transport; secretion |
| seq0017 181448 |
C→T | S97F (S72F)b |
D512_21859 (BPSS0946) |
NS; neutral | Yes | Class A β-lactamase PenA |
β-Lactam resistance |
| seq0020 32593 |
C→A | F99L |
D512_23016 (BPSS1260) |
NS; neutral | No | Hypothetical protein | Unknown |
| seq0028 205054 |
G→T | R458L |
D512_25718 (BPSS1501) |
NS; neutral | Noc | ClpB | Heat shock |
| seq0028 229189 |
T→C | H322R |
D512_25813 (BPSS1520) |
NS; neutral | Yesd | AraC regulator | DNA regulation, virulence |
| seq0028 480279 |
T→C | D54G |
D512_26783 (BPSS1698) |
NS; deleterious | Yes | RsrI | DNA methylation |
| seq0028 553993 |
C→T | A660T |
D512_27123 (BPSS1755) |
NS; deleterious | Yes | RpoD | Primary sigma factor |
| seq0028 1047356 |
G→A | NA | NA | NA | No | NA | Unknown |
| seq0028 1280562 |
G→A | S2S |
D512_30428 (BPSS2321) |
S; neutral | Yes | Hypothetical protein | Unknown |
b
Resides within the previously described, conserved PenA 70SXXK73 motif (68). S72F causes ~4-fold increased resistance to amoxicillin-clavulanic acid but not to other β-lactams (20, 69).
c
MSHR1655 possesses a different mutation in this gene (insertion of a repetitive NAP motif at the 3′ end).
d
MSHR1043 possesses the mutant allele; MSHR1655 and MSHR6686 possess the wild-type allele.
e
NA, not applicable.