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Electron micrographs of immunogold-labeled bundled pili on strains RM11.2nv and RM11.2nv mpg (Δ1686). Representative electron micrographs are shown for strain RM11.2nv (parent) and RM11.2nv mpg (Δ1686). Bars, 0.5 µm (A, B, D, and E) and 200 nm (C and F). Pili were detected using a rabbit antipeptide polyclonal antiserum directed against the RM11.2 hypervariable pilin sequence and a gold-labeled secondary antibody.
