Table 1. Effects of PTEN on gene expression in PC12 cells.
| Accession no.
|
Fold change
|
||||
|---|---|---|---|---|---|
| Gene name | Microarray | Q-PCR | P value* | Description | |
| Tyrosine hydroxylase | M10244 | −3.9 | −104.2 | 4 × 10−4 | Key dopamine synthesis enzyme |
| GTP cyclohydrolase I | M58364 | −5.1 | −7.1 | 0.025 | Key dopamine synthesis enzyme |
| Trk A | M85214 | −2.5 | −4.4 | 0.035 | High-affinity NGF receptor |
| P75 | X05137 | −4.7 | −18.9 | 0.039 | Low-affinity NGF receptor |
| 5-HT3 receptor | D49395 | −3.0 | −530.2 | 3 × 10−5 | Serotonin receptor |
| GABA(A) receptor β-3 subunit | X15468 | −17.3 | −182.8 | 3 × 10−5 | Part of the GABA receptor complex |
| PACAP receptor | D14909 | −3.6 | −666.7 | 3 × 10−5 | Receptor for pituitary adenylate cyclase-activating polypeptide |
| Bone morphogenetic protein 7 | D29769 | −24.2 | −57.8 | 10−4 | Multiple roles in neural development and plasticity |
| SHIP | U55192 | 0.92 | 81.9 | 7 × 10−3 | Src homology 2 domain-containing inositol 5′-phosphatase |
| Fez1 | U63740 | −60.56 | −380.7 | 2 × 10−4 | Regulation of axonal outgrowth |
| SNAP-25 | AB003991 | −13.2 | −38.5 | 6 × 10−5 | Regulation of exocytosis in neurons |
| SNAP-23 | AF052596 | 7.37 | 2.9 | 0.019 | Regulation of exocytosis, non-neuronal homologue of SNAP-25 |
*
P values for each gene were determined by two-tail t test comparing all four control (C1-C4) and PTEN (P1-P4) cell lines.