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tPA expression in the NAcc after morphine treatment. In situ hybridization analysis of tPA mRNA expression in the NAcc of saline-treated wild-type mice (A), morphine-treated wild-type mice (B), and morphine-treated tPA-/- mice (C). (D-F) Immunohistochemical analysis of morphine-induced tPA expression in the NAcc of mice. (D) MAP2. (E) tPA. (F) Merge. (Bar = 20 μm.)
