FIG 5 .

BamA^Ecoli loops 6 and 7 are required for CDI^EC93. (A and B) Competitions between E. coli EC93 and E. coli ∆bamA::cat targets that express the indicated bamA^Ecoli alleles. For all competitions, wild-type E. coli EC93 (cdiA⁺) or E. coli EC93 ∆cdiA mutant cells were cultured with target bacteria at a 1:1 ratio in LB broth for 4 h. Cultures were sampled at 0 and 4 h and plated on LB agar supplemented with ampicillin to enumerate viable target cells as CFU per milliliter. (C) Adhesion between CDI^EC93 inhibitor and target cells. GFP-labeled E. coli MC4100 cells carrying cosmid pDAL660∆1-39 (CDI^EC93 +) or pWEB::TNC (CDI^EC93 −) were used as inhibitors and mixed in 5-fold excess with DsRed-labeled target bacteria expressing the indicated bamA^Ecoli alleles. Cell suspensions were analyzed by flow cytometry, and the percentage of red-fluorescent target cells aggregated with green-fluorescent inhibitors was calculated. The reported values are the mean ± the standard error of the mean from two independent experiments.