TABLE 1 .
Strains used in this study, antimicrobial susceptibility, topoisomerase genotype and relative fitnessa
| Strain | GyrA substitution | Relative fitnessb | MIC (µg/ml) |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Cip | Nal | Chl | Tet | Kan | Gent | Amp | Tric | EtBr | |||
| SL1344 | None (wild type) | 1.00 (0.02) | 0.03 | 4 | 2 | 1 | 2 | 0.5 | 0.5 | 0.06 | >512 |
| L821 | Ser83Phe | 0.96 (0.04) | 0.5 | >256 | 4 | 1 | 2 | 0.5 | 1 | 0.25 | >512 |
| L825 | Asp87Gly | 0.92 (0.03) | 0.5 | >256 | 4 | 1 | 2 | 0.5 | 1 | 0.25 | >512 |
a
Cip, ciprofloxacin; Nal, nalidixic acid; Chl, chloramphenicol; Tet, tetracycline; Kan, kanamycin; Gent, gentamicin; Amp, ampicillin; Tric, triclosan; EtBr, ethidium bromide.
b
Defined as 1 + S (the selection coefficient). Numbers in parentheses are standard deviations.