TABLE 2 .
Cell membrane phospholipid composition and asymmetry of Enterococcus faecalis S613 and derivatives
| Strain | Cell membrane PL composition (mean % ± SD)a |
|||||||
|---|---|---|---|---|---|---|---|---|
| Positively charged PLb |
Total ACPc | Negatively charged PLd |
||||||
| I-LPG | O-LPG | PG | CL | GP-DGDAG | PA | NegPL | ||
| S613 | 6.81 ± 2.66 | 6.52 ± 1.09 | 19.51 ± 4.99 | 20.76 ± 0.11 | 44.0 ± 3.99 | 5.07 ± 1.05 | 4.13 ± 0.01 | 6.54 ± 0.06 |
| S613∆liaF177gdpD170cls61 | 5.48 ± 1.09 | 10.24 ± 1.45 | 27.14 ± 2.62 | 13.22 ± 1.65 | 30.85 ± 1.30 | 9.58 ± 2.05 | 9.27 ± 0.10 | 9.94 ± 0.33 |
| S613∆liaF177gdpD170 | 5.58 ± 2.44 | 7.36 ± 0.62 | 22.63 ± 2.96 | 17.95 ± 0.86 | 39.42 ± 3.26 | 6.77 ± 0.10 | 5.52 ± 0.40 | 7.71 ± 0.85 |
| S613∆gdpD170 | 6.34 ± 0.79 | 7.56 ± 0.42 | 21.63 ± 0.07 | 18.09 ± 0.81 | 40.11 ± 1.88 | 6.35 ± 0.77 | 5.40 ± 1.03 | 8.42 ± 1.79 |
| S613∆liaF177 | 6.09 ± 0.25 | 6.83 ± 2.19 | 19.90 ± 1.64 | 19.95 ± 2.11 | 40.74 ± 2.38 | 6.96 ± 1.12 | 4.72 ± 0.50 | 7.72 ± 1.23 |
Values that are statistically significantly different (P < 0.05) from the values for strain S613 are shown in boldface type. PL, phospholipid.
LPG, lysyl-phosphatidylglycerol; I-LPG, LPG in the inner leaflet of the cell membrane; O-LPG, LPG in the outer leaflet of the cell membrane. The amount of phospholipid flipping is determined by the difference between LPG content in the inner and outer leaflets of the cell membrane.
ACP, amino-containing phospholipid. The amount of total ACP includes O-LPG, I-LPG, and two additional ACPs whose identity has not been established.
Major anionic (negatively charged) phospholipid species isolated from the membrane of E. faecalis strains. PG, phosphatidylglycerol; CL, cardiolipin; GP-DGDAG, glycerolphospho-diglycodiacylglycerol; PA, phosphatidic acid; NegPL, a negatively charged phospholipid species whose structure has not been determined.