TABLE 1 .
Identification of MagD partners as revealed by immunoprecipitation coupled with mass spectrometry analysesa
| Locus tagb |
Gene name |
Product name | Mean SC of PAO1 ΔretS |
Mean SC of PAO1 ΔretS ΔmagD |
Enrichment |
|---|---|---|---|---|---|
| PA4491 | magB | MagB | 86.5 | 2.5 | 34.6 |
| PA2462 | DUF637 (possible hemagglutinin) |
63 | 2 | 31.5 | |
| PA4487 | magF | MagF | 34.5 | 1.5 | 23 |
| PA3068 | gdhB | NAD-dependent glutamate dehydrogenase |
30 | 0.5 | 60 |
| PA4492 | magA | MagA | 19 | 0 | wt only |
| PA0077 | tssM1 | TssM1 | 19 | 0 | wt only |
| PA5304 | dadA |
d-Amino acid dehydrogenase, small subunit |
18 | 0 | wt only |
| PA2953 | Electron transfer flavoprotein ubiquinone oxidoreductase |
16.5 | 1 | 16.5 |
a
Proteins immunoprecipitated from the parental and ΔmagD strains with anti-MagD antibodies were analyzed using shotgun proteomics. Proteins identified as having at least 10 spectral counts (SC) in two biological replicates, either only in the parent or enriched at least 10 times (based on SC) in the parent over the ΔmagD strain, are listed. Proteins encoded by the same operon as MagD are boldface.
b
From http://www.pseudomonas.com. The whole list of identified proteins is provided in the supplemental material.