TABLE 1 .
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype and phenotype | Source |
|---|---|---|
| V. cholerae strains | ||
| O395 | Classical Ogawa, Smr | Laboratory collection |
| E7946 | El tor, Smr | Laboratory collection |
| RA25 | O395 ΔtoxR | 3 |
| RA6 | E7946 ΔtoxR | This work |
| NB39 | O395 + ptoxT-GFP | Laboratory collection |
| NB40 | O395 ΔtoxR + ptoxT-GFP | Laboratory collection |
| RA179 | O395 ΔtoxT | 43 |
| RA67 | O395 ΔtcpP | 44 |
| RA305 | O395 ΔaphA | This work |
| RA282 | O395 ΔaphB | This work |
| RA286 | O395 + pMT5 | 45 |
| RA289 | O395 + pMMB66EH-aphA | This work |
| RA290 | O395 + pMMB66EH-aphB | This work |
| RA306 | O395 ΔaphA + pMMB66EH | This work |
| RA307 | O395 ΔaphA + pMMB66EH- aphA |
This work |
| RA308 | O395 ΔaphB + pMMB66EH | This work |
| RA309 | O395 ΔaphB + pMMB66EH- aphB |
This work |
| Plasmids | ||
| ptoxT-GFP | pBH6119-toxTpro-gfp | This work |
| pMT5 | pMMB66HE-toxT | 45 |
| pWM91-ΔaphA | pWM91-ΔaphA | 46 |
| pWM91-ΔaphB | pWM91-ΔaphB | 46 |
| pMMB66EH- aphA |
pMMB66EH-aphA (O395) | This work |
| pMMB66EH- aphB |
pMMB66EH-aphB (O395) | This work |