FIG 3 .

Impact of METH on C. neoformans capsular polysaccharide released in vitro. (A) Capsule size measurements of C. neoformans strain H99 cells were done for cells grown in the absence and presence of 25 and 50 µM/ml METH. The capsule size of 100 cells was measured. (B) GXM concentration in the supernatant of C. neoformans cultures coincubated in the absence or presence of METH. (A and B) Averages and standard deviations were determined. Asterisks denote P value significance (*, P < 0.05; **, P < 0.001) calculated by analysis of variance and adjusted by use of the Bonferroni correction. (C) Representative India ink images displaying the effect of METH on the capsule size of C. neoformans. Untreated fungal cells exhibited larger capsules than METH-treated C. neoformans cells. The pictures were taken using a ×100-power field. Bar, 2 µm. The experiments were performed twice, and similar results were obtained.