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. 2013 Jul 30;4(4):e00400-13. doi: 10.1128/mBio.00400-13

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Copyright © 2013 Patel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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FIG 4 — METH alters the size of C. neoformans capsular polysaccharide fractions. Capsular polysaccharide obtained from untreated (A) and METH-treated (B) strain H99. The x axis represents size distribution by particle diameter; the y axis corresponds to the values of percentage intensity-weighted sizes obtained with the nonnegative least-squares (NNLS) algorithm. (C) METH promotes capsular polysaccharide release from cryptococcal cells. After coincubation with 25 µM METH, the cells were washed and incubated with Uvitex and MAb 18B7-FITC-conjugated goat anti-mouse IgG1 stain to label the cell wall (blue) and capsular polysaccharide (green), respectively. Bar, 2 µm.