TABLE 1 .
Glycosyl composition analysis of capsular polysaccharide released in culture medium and isolated from untreated or METH-treated C. neoformans strain H99
| Glycosyl residue | Untreated |
25 µM METH |
||
|---|---|---|---|---|
| Mass (mg) | Mol%a | Mass (mg) | Mol% | |
| Ribose (Rib) | 3.0 | 1.0 | 2.1 | 0.5 |
| Arabinose (Ara) | NDb | ND | ||
| Rhamnose (Rha) | ND | ND | ||
| Fucose (Fuc) | ND | ND | ||
| Xylose (Xyl) | 88.3 | 28.1 | 134.9 | 30.0 |
| Glucuronic acid (GlcA) | 45.8 | 11.3 | 63.6 | 10.9 |
| Galacturonic acid (GalA) | ND | ND | ||
| Mannose (Man) | 201.4 | 53.4 | 247.3 | 45.8 |
| Galactose (Gal) | 9.1 | 2.4 | 6.6 | 1.2 |
| Glucose (Glc) | 14.1 | 3.7 | 61.7 | 11.4 |
| N-acetylgalactosamine (GalNAc) | ND | ND | ||
| N-acetylglucosamine (GlcNAc) | 1.0 | 0.2 | 0.6 | 0.1 |
| N-acetylmannosamine (ManNAc) | ND | ND | ||
| Sum of carbohydrates | 359.6 | 514.5 | ||
a
Values are expressed as mole percentages of total carbohydrate. The total percentage may not add up to exactly 100% due to rounding.
b
ND, not detected. Samples for two independent experiments were analyzed, with similar results.