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. 2013 Aug 6;4(4):e00360-13. doi: 10.1128/mBio.00360-13

FIG 5 .

FIG 5

The strain M2 PilD homolog acted as a prepilin peptidase. Whole-cell lysates of the M2∆pilA::kan(pilA-FLAG+) and M2∆pilA::kan∆pilD::strAB(pilAFLAG+) strains were examined by Western blot analysis for processed and unprocessed PilA-FLAG. The nonspecific band around 50 kDa was included to demonstrate equal migration of proteins. PilA-FLAG from the M2∆pilA::kan(pilA-FLAG+) migrated to a slightly lower position than PilA-FLAG from M2∆pilA::kan∆pilD::strAB(pilAFLAG+). The leader peptide of PilA is predicted to be six amino acids.