TABLE 1.
SCHEME OF THE EXPERIEMENT
| Regimens* | Number of mice used to determine lung CFU counts (number of mice held for relapse after treatment completion)† |
||||||
|---|---|---|---|---|---|---|---|
| D−13 | D0 | M2 | M4 | M5 | M6 | M7 | |
| Control groups |
|
|
|
|
|
|
|
| Infected, untreated |
12 |
20 |
+10 monitored for survival |
||||
| 2 mo RHZ + RH |
|
|
5 |
5 |
5 (30) |
5 (30) |
|
| Test groups |
|
|
|
|
|
|
|
| M alone |
|
|
5 |
5 |
5 |
|
|
| L alone |
|
|
5 |
5 |
5 |
|
|
| 2 mo MEtZA + MEt |
|
|
5 |
5 |
5 |
|
|
| 2 mo MEtZA + MEtZ |
|
|
|
5 |
5 (30) |
5 (30) |
5 (30) |
| 2 mo LEtZA + LEt |
|
|
5 |
5 |
5 |
|
|
| 2 mo LEtZA + LEtZ | 5 | 5 (30) | 5 (30) | 5 (30) | |||
Definition of abbreviations: A = amikacin; CFU = colony-forming unit; Et = ethionamide; H = isoniazid; L = levofloxacin; M = moxifloxacin; R = rifampin; Z = pyrazinamide.
*
Drugs were administered orally, 5 d/wk, at the following doses: R at 10 mg/kg, H at 25 mg/kg, Z at 150 mg/kg, M at 100 mg/kg, L at 300 mg/kg, and Et at 50 mg/kg. A was administered subcutaneously at 100 mg/kg. Mice were killed at the following times: 1 d after infection (D−13); 14 d after infection (D0); and after 2, 4, 5, 6, and 7 mo of treatment (M2, M4, M5, M6, and M7, respectively).
†
Numbers in parentheses indicate that 30 mice were killed 3 mo after completing the indicated duration of treatment to assess for relapse.