Table 1. Proteins with a threefold difference in expression in round-headed and normal sperm as identified by Matrix-assisted laser desorption/ionization-Time of Flight/Time of Flight*.

Spot	Protein name	IPI	Mr	Score	Sequence	Change in regulation coverage in round-headed sperm
1	A-kinase (PRKA) anchor protein 4	157 860	95 842	234	9	Up
2	Clusterin precursor	291 262	53 031	205	9	Up
3	ALB protein	216 773	46 442	74	2	Up
4	Testis-specific gene A2 protein	103 777	35 159	62	4	Up
5	Sperm protein	22 057	97 407	230	9	Up
6	Glutathione S-transferase Mu 3	246 975	26 998	154	15	Down
7	Triosephosphate isomerase 1 variant	465 028	31 057	374	26	Down
8	Isoform 1 of triosephosphate isomerase	797 687	26 938	347	24	Down
9	Isoform 2 of triosephosphate isomerase	451 401	27 451	244	19	Down
10	30 kDa protein	746 832	29 976	237	19	Down
11	22 kDa protein	796 633	22 711	237	17	Down
12	RcTPI1 (fragment)	383 071	27 211	128	13	Down
13	Actin, cytoplasmic variant 1	21 439	42 052	157	14	Up
14	Actin, cytoplasmic variant 2	21 440	42 108	157	14	Up
15	ACTG1 protein	794 523	28 478	114	10	Up
16	Actin-related protein T2	154 776	42 074	94	11	Down
17	Outer dense fibre of sperm tails 2 isoform 2	642 221	73 977	75	14	Down
18	Outer dense fibre of sperm tails 2 isoform 1	171 245	93 823	71	15	Down
19	Outer dense fibre of sperm tails 2	643 280	89 161	69	14	Down
20	Outer dense fibre protein 2	1 758	96 140	81	18	Down
21	Prolactin-inducible protein precursor	22 974	16 847	127	11	Up
22	Isoform 2 of triosephosphate isomerase	451 401	27 451	66	1	Down
23	94 kDa protein	22 057	96 072	38	2	Down
24	Isoform 1 of A-kinase anchor protein 4 precursor	157 860	95 842	119	2	Down
25	Isoform 2 of A-kinase anchor protein 4 precursor	333 264	94 811	119	2	Down
26	Asparaginase-like 1 protein	555 734	32 406	140	2	Down
27	Isoform 1 of tubulin α-2 chain	179 709	50 612	43	1	Down
28	Isoform 2 of tubulin α-2 chain	218 345	46 935	43	1	Down
29	Similar to α-tubulin	410 402	50 568	43	1	Down
30	α-tubulin isotype H2-α	552 356	50 615	43	1	Down
31	46 kDa protein	743 604	47 147	43	1	Down
32	Sperm protein associated with the nucleus C on the X chromosome	6 961	11 052	47	1	Down
33	Sperm protein associated with the nucleus on the X chromosome A	186 783	11 088	47	1	Down
34	SPANX-B	760 825	11 834	47	1	Down
35	Sperm acrosome membrane-associated protein 1 precursor	8 910	32 693	168	3	Down

Abbreviations: IPI, international protein index; Mr, relative molecular weight.

^*The spot number listed in this table is not in accordance with the spot number listed in Figure 3.