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. 2009 Oct 5;11(6):731–739. doi: 10.1038/aja.2009.66

Figure 3.

Figure 3

Localization of EPPIN (epididymal protease inhibitor) protein in rat testis and epididymis. Testis and epididymis from adult rat were fixed in 4% paraformaldehyde, paraffin embedded and sectioned for immunohistochemistry analysis. Sections stained with Eppin (I-12) probe. In the testis, (A and B) the labels were primarily in the elongated spermatids (ES). In the cauda epididymis (D and E), the labels were primarily in the epithelial cells (EC) and spermatozoa (SZ). No signal was seen in the testis (C) and cauda epididymis (F) when the primary antibody was neutralized with recombinant human EPPIN. (D): scale bar = 100 μm; (A, B, C, E and F): scale bars = 25 μm.