Figure 4.

Detection of Eppin (epididymal protease inhibitor) mRNA in primarily cultured Sertoli cells. Sertoli cells were isolated from adult testis and primarily cultured. The mRNA was extracted for Reverse transcriptase PCR (RT-PCR) analysis. A 308-bp band predicted for rat Gapdh was seen in the PCR product using Sertoli cell complementary DNA (cDNA) as templates. However, it did not produce any band when primers specific for Eppin's coding sequence were added. The testis cDNA prepared as described in Section 2.2 and deionized water were used as positive and negative control, respectively.