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. Author manuscript; available in PMC: 2013 Dec 15.
Published in final edited form as: J Immunol. 2012 Nov 14;189(12):5590–5601. doi: 10.4049/jimmunol.1201744

Figure 1. PAP antigen-specific T effector immune responses following immunization are suppressed by PAP-specific T regulatory cells in a CTLA-4-dependent fashion.

Figure 1

Panel A. IFNγ ELISPOT responses of patient ID007 prior to (Pre) or two weeks following (Post) six biweekly immunizations, and three-month intervals thereafter. PBMC were evaluated for IFNγ responses following stimulation with media alone (No Ag, grey), PAP (black), tetanus toxoid (TT/D, vertical hatches), or a PHA positive control (horizontal hatches). Shown are the mean and standard deviation of quadruplicate samples in spot-forming units (sfu). Panel B. Pre- (grey) or post-immunization (black) PBMC from three patients (ID005, ID007, and ID014) were injected into the footpads of SCID mice with the indicated antigens (TT/D, PAP, or PSA) and blocking antibodies (IgG control, anti-CTLA-4, anti-TGFβ, or anti-IL-10). DTH swelling responses (10−4 in.) were measured after 24 hours. Data shown are representative of at least two independent experiments. Differences in mean tvDTH between groups were analyzed by student’s t-test, with * indicating p<0.05. Panel C. Post-immunization samples from patient ID005 and ID014 were used in tvDTH assays measuring responses to TT/D alone or in combination with PAP or PSA, and in the presence or absence of a CTLA-4 blocking antibody. Data shown are representative of at least two independent experiments. Differences between mean DTH were compared using a student’s t-test, with * indicating p<0.05.