Figure 6. PAP-specific CD8+ regulatory responses mediate tvDTH suppression via IL-35.
PBMC from patients with CTLA-4-regulated PAP-specific bystander suppressive immune responses were stimulated with TT/D alone or TT/D and PAP in the presence of either an IgG control or blocking antibodies directed against CTLA-4, IL-12p35, IL-12p40, IL-23p19, IL-27p28, Ebi3, a combination of IL-12p35 and Ebi-3 (both IL-35 subunits), IL-10, or TGFβ. Data shown are representative of at least two independent experiments. Differences between treatment group mean DTH values (indicated by solid lines) were compared using a student’s t-test, with * indicating p<0.05.