Fig. 3.
The effects of IPostC on infammatory cell infiltration in the ischemic brain 3 days after stroke. A. Gating strategies to identify immune and microglial cells in the ischemic brain. Both microglial and leukocytes are identified by expression of CD45 antigen. Microglia is identified as CD45intCD11b+ cells, while monocyte-derived macrophages are defined as CD45hiCD68+ cells. CD4 T cells, CD8 T cells and B cells are identified as CD45+CD4+, CD45+CD8+ and CD45+CD19+ cells, respectively. B. Mean and SD of individual subsets for each group, *P<0.05 and **P<0.01 between naive and sham groups; #P<0.05 and ##P<0.01 compared to control group. n=5 mice/group.