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. Author manuscript; available in PMC: 2013 Aug 6.
Published in final edited form as: Cell Metab. 2011 Oct 5;14(4):504–515. doi: 10.1016/j.cmet.2011.07.013

Figure 2. Among PC Synthesis Enzymes, CCT Uniquely Localizes to LDs.

Figure 2

(A) Overview of the reactions of the Kennedy pathway.

(B) Transiently expressed mCherry-tagged CCT1 and CCT2, but not CK and CPT, (left panels) localize to LDs in S2 cells loaded with 1 mM oleate for 12 hr. Overlays and a magnification are shown (right two panels). Bar, 5 μm (overview) or 1μm (magnification).

(C) Endogenous CCT localizes to LDs. Normalized peptide intensities determined by LC-MS/MS are shown for fractions of a LD purification. CCT, hormone-sensitive lipase (HSL, LD marker), disulfide isomerase (PDI; ER marker), lamin (nuclear marker), cytochrome oxidase subunit Va (CoVa; mitochondrial marker), and aldehyde dehydrogenase (ALDH; cytosol marker) are shown. Bottom panels show western blots for CCT1, KDEL receptor, and Gapdh and a TLC for TG of each fraction normalized to protein.