Figure 3.

A. fumigatus conidia-induced recruitment of neutrophils to the conducting airways of mice with AAI. Representative confocal images taken from the distal ventral segment of conducting airway at 2 hours post-conidial inhalation by (A) OVA/PBS and (B) OVA/OVA mice. Images are represented as Z- (upper panels) and Y-projection (lower panels). Ly-6G⁺ neutrophils are shown in green and epithelial auto-fluorescence in dark green. Epithelial and subepithelial compartments, separated by a dashed line, were determined based on epithelial auto-fluorescence (lower panels). Neutrophil in the epithelial compartment of the conducting airway mucosa is indicated by arrow. The images are shown as MIP and the scale bar = 50 μm.