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. 2013 Jul 27;14(1):78. doi: 10.1186/1465-9921-14-78

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Copyright © 2013 Shevchenko et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Internalization of A. fumigatus conidia in the epithelial compartment of conducting airways. (A) Representative confocal image taken from the proximal ventral segment of the whole-mount airway main axial pathway showing the distribution of MHC II⁺ epithelial DCs (upper left panel: MHC II⁺ DCs, yellow; epithelium auto-fluorescence, dark yellow), Ly-6G⁺ neutrophils (upper right panel, green), A. fumigatus conidia (lower left panel, red), and a merged image (lower right panel) at 4 hours post-conidial application. Scale bar = 20 μm. (B, C) The neutrophil locating in close proximity to the luminal side of the epithelium (arrowhead) and the intraepithelial DC (arrow) are indicated on Z-projection (left panels) or on X-projection (right panels) of the area displayed in (A). (D) The same neutrophil and DC are displayed on the three-dimensional larger magnification image of the region boxed in (A). Scale bar = 20 μm (A, B, C); and 10 μm (D).