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. 2013 May 22;33(21):9056–9067. doi: 10.1523/JNEUROSCI.3870-12.2013

Figure 4.

Figure 4.

Electrical recordings and neuronal counts confirm that LY 294002 (LY) and rapamycin (RAPA) treatments reduce number of ictal events and promote neuronal survival. A, Typical ictal event in an epileptic control culture. B, Representative electrical recordings from control, rapamycin, and LY 294002-treated cultures. Rapamycin and LY 294002 treatments reduce seizure frequency (C), but not seizure duration (D), with the overall effect of reducing the time spent in ictal state (E). **p < 0.01, ***p < 0.001. F, Representative images of Nissl-stained organotypic hippocampal cultures at 28 DIV. Scale bars, 50 μm. G, Neuronal counts revealed that more neurons survive in CA3c and CA1 of rapamycin and LY 294002-treated cultures compared with epileptic controls, n = 3 per condition, **p < 0.01, ***p < 0.001.