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. Author manuscript; available in PMC: 2013 Aug 6.
Published in final edited form as: J Membr Biol. 2012 Jul 24;245(8):453–463. doi: 10.1007/s00232-012-9457-z

Fig. 2.

Fig. 2

TM3 does not contribute to the pore in open Cx46 hemichannels. Shown are effects of Cys substitutions for TM3 residues F157 through F175 (left) and SCAM results using MTSET (right). For the Cys substitutions, the change in unitary conductance represents the mean percentage change in the slope conductance compared with wt Cx46 measured at Vm = 0 from fitted open channel I–V relations. Of the 19 residues examined, only F163CF failed to form functional hemichannels and single hemichannels were not observed from oocytes injected with K162C due to consistently low expression levels. For the SCAM results, the change in unitary conductance represents the mean percentage change in the slope conductance relative to the Cys substituted mutant measured at Vm = 0 from fitted open channel I–V relations. No substantial changes were observed at any positions. NC denotes nonfunctional hemichannels. ND-MACRO denotes residues for which there was insufficient single-channel data for characterization but macroscopic data in ND96 with 2mM Ca2+ and 0.2mM Ca2+ showed no reactivity. Error bars represent standard deviations; n ≥ 8 for each mutant.