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. Author manuscript; available in PMC: 2013 Aug 6.
Published in final edited form as: J Membr Biol. 2012 Jul 24;245(8):453–463. doi: 10.1007/s00232-012-9457-z

Fig. 3.

Fig. 3

SCAM of select TM3 residues using a large MTS reagent shows no evidence of reactivity. Shown is an alignment of Cx32, Cx46 and Cx50 sequence from the CL/TM3 border through the proximal portion of E2. Residues indicated by arrows in Cx32 were reported to be pore-lining in Cx32 GJ channels (Skerrett et al., 2002). Boxed residues in Cx46 and Cx50 were subjected to SCAM analysis using MTS biotin-X. These include Y155, I159, F161, V167, F169 and A171 in Cx46 and Y158, V170 and F172 in Cx50. No effects were observed at any of these positions.