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Unitary conductances of Cx46 and Cx50 hemichannels are specified by NT, E1 and TM1 domains. Shown is a bar graph summarizing unitary conductances of WT Cx46 (blue Cx) and WT Cx50 (red Cx). Unitary conductances represent slope conductances at Vm = 0 from fits to open channel I–V relations obtained by applying 8 sec ramps to patches containing single hemichannels. Sequence substituted in chimeras is indicated by color. Arrows denote substitutions of just NT domains.
