Figure 2.

Withaferin A (WA) administration inhibits incidence of lung metastasis in mouse mammary tumor virus–neu mice. A) Representative hematoxylin and eosin–stained lung sections exhibiting metastasis in representative mice of the WA treatment group and the control group (×100 magnification, scale bars = 200 µm). B) Incidence of lung metastasis. Results shown are the percentages of mice with lung metastasis. The P value was calculated by two-sided Fisher exact test. C) Multiplicity of lung metastasis. Results show mean number of lung metastasis per mouse with 95% confidence intervals (error bars). Statistical significance of differences was determined by two-sided Student t test. D) Area of lung metastatic foci. Results represent the mean areas of lung metastatic tumor with their corresponding 95% confidence intervals (error bars). Statistical significance of differences was determined by two-sided Student t test.