Table 1.
Identification of altered proteins in the tumors of mouse mammary tumor virus–neu (MMTV-neu) mice in response to withaferin A treatment
| Protein name | Master No. | Protein ID | Average ratio, withaferin A/control* | P† |
|---|---|---|---|---|
| Upregulation | ||||
| Matr3 [Mus musculus] | 165 | 2 | 1.4 | .08 |
| Gelsolin isoform 2 [Mus musculus] | 312 | 4 | 2.0 | .07 |
| Moesin [Mus musculus] | 444 | 5 | 1.4 | .08 |
| Acyl-Coenzyme A dehydrogenase, very long chain [Mus musculus] | 551 | 7 | 1.4 | .07 |
| Sorting and assembly machinery component 50 homolog [Mus musculus] | 741 | 9 | 1.6 | .02 |
| Protein synthesis initiation factor 4A [Mus musculus] | 900 | 11 | 1.4 | .06 |
| Alpha-fetoprotein [Mus musculus] | 1066 | 12 | 1.8 | .08 |
| Annexin A1 [Mus musculus] | 1134 | 20 | 1.4 | .04 |
| Annexin A2 [Mus musculus] | 1173 | 21 | 1.3 | .08 |
| Anxa5 protein [Mus musculus] | 1302 | 24 | 1.4 | .09 |
| Put. β-actin (aa 27–375) [Mus musculus] | 1328 | 27 | 2.6 | .10 |
| Protein disulfide-isomerase A3 precursor [Mus musculus] | 1341 | 29 | 2.5 | .07 |
| Enoyl-CoA hydratase domain-containing protein 1 isoform b [Mus musculus] | 1306 | 30 | 2.6 | .07 |
| Esterase D/formylglutathione hydrolase, isoform CRA_b [Mus musculus] | 1310 | 31 | 1.3 | .02 |
| U2 small nuclear ribonucleoprotein A′ [Mus musculus] | 1482 | 32 | 2.4 | .07 |
| Sepiapterin reductase [Mus musculus] | 1580 | 33 | 1.5 | .08 |
| NADH dehydrogenase (ubiquinone) Fe-S protein 3 [Mus musculus] | 1590 | 34 | 2.8 | .08 |
| Rexo2 protein [Mus musculus] | 1624 | 35 | 1.6 | .001 |
| Growth factor receptor bound protein 2 [Mus musculus] | 1628 | 36 | 1.6 | .07 |
| Proteasome subunit beta type-3 [Mus musculus] | 1650 | 37 | 1.5 | .01 |
| Triosephosphate isomerase [Mus musculus]‡ | 1675 | 39 | 1.3 | .09 |
| RIKEN cDNA 0610011F06, isoform CRA_b [Mus musculus] | 1695 | 40 | 2.5 | .09 |
| RecName: Full=UMP-CMP kinase; AltName:Full=Cytidine monophosphate kinase; AltName: Full=Cytidylate | 1711 | 41 | 1.5 | .08 |
| Ferritin light chain 2 [Mus musculus] | 1731 | 42 | 3.5 | .06 |
| GTP-binding protein SAR1a [Mus musculus] | 1715 | 43 | 1.5 | .01 |
| Cell division control protein 42 homolog isoform 1 [Homo sapiens] | 1756 | 44 | 1.5 | .02 |
| Chain A, Recombinant Mouse L-Chain Ferritin | 1755 | 45 | 1.6 | .01 |
| Alpha-S2-casein-like A precursor [Mus musculus] | 1803 | 46 | 4.4 | .10 |
| Vacuolar protein sorting-associated protein 29 [Mus musculus] | 1804 | 47 | 1.3 | .01 |
| Vacuolar protein-sorting-associated protein 25 [Mus musculus] | 1871 | 48 | 1.9 | .03 |
| Ferritin heavy chain [Mus musculus]‡ | 1800 | 49 | 2.0 | .02 |
| Ferritin heavy chain [Mus musculus]‡ | 1795 | 50 | 2.5 | .07 |
| c-Myc-binding protein [Mus musculus] | 2411 | 65 | 1.3 | .09 |
| Downregulation | ||||
| Damage-specific DNA binding protein 1 [Mus musculus] | 168 | 1 | –1.4 | .003 |
| Pyruvate carboxylase, mitochondrial isoform 2 [Mus musculus] | 175 | 3 | –1.4 | .06 |
| Transketolase [Mus musculus] | 540 | 6 | –1.3 | .01 |
| M2-type pyruvate kinase [Mus musculus] | 640 | 8 | –1.4 | .04 |
| Calreticulin precursor [Mus musculus] | 742 | 10 | –1.4 | .003 |
| Isocitrate dehydrogenase 1 (NADP+), soluble [Mus musculus] | 944 | 13 | –1.3 | .003 |
| Phosphoglycerate kinase [Mus musculus] | 969 | 14 | –1.5 | .06 |
| Creatine kinase U-type, mitochondrial precursor [Mus musculus] | 1003 | 15 | –2.1 | .09 |
| Acetyl-CoA acetyltransferase, mitochondrial precursor [Mus musculus] | 1026 | 16 | –1.5 | .09 |
| Fructose-bisphosphate aldolase A isoform 2 [Mus musculus]‡ | 1041 | 17 | –1.7 | .03 |
| Fructose-bisphosphate aldolase A isoform 2 [Mus musculus]‡ | 1038 | 18 | –1.7 | .02 |
| Phosphoserine aminotransferase isoform 2 [Mus musculus] | 1109 | 19 | –1.4 | .02 |
| Glyceraldehyde-3-phosphate dehydrogenase [Mus musculus] | 1184 | 22 | –1.8 | .045 |
| Heterogeneous nuclear ribonucleoproteins A2/B1 isoform 1 [Mus musculus] | 1255 | 23 | –1.3 | .08 |
| Eukaryotic translation elongation factor 1 beta 2 [Mus musculus] | 1419 | 25 | –1.4 | .02 |
| Impa1 protein [Mus musculus] | 1368 | 26 | –1.4 | .01 |
| Proteasome activator PA28 beta subunit [Mus musculus] | 1407 | 28 | –1.3 | .01 |
| Triosephosphate isomerase [Mus musculus]‡ | 1609 | 38 | –1.6 | .06 |
| Myosin light chain, regulatory B-like [Mus musculus]‡ | 1864 | 51 | –1.9 | .01 |
| Myosin light chain, regulatory B-like [Mus musculus]‡ | 1860 | 52 | –1.6 | .01 |
| Neurocalcin-delta [Bos taurus] | 1955 | 53 | –1.7 | .04 |
| Bis(5′-nucleosyl)-tetraphosphatase [asymmetrical] [Mus musculus] | 2134 | 54 | –2.5 | .01 |
| PREDICTED: peptidyl-prolyl cis-trans isomerase A-like [Mus musculus]‡ | 2138 | 55 | –2.2 | .004 |
| Tumor metastatic process-associated protein NM23 [Mus musculus] | 2200 | 56 | –1.7 | .02 |
| Nucleoside diphosphate kinase B [Mus musculus] | 2124 | 57 | –1.4 | .06 |
| PREDICTED: peptidyl-prolyl cis-trans isomerase A-like [Mus musculus]‡ | 2175 | 58 | –1.5 | .001 |
| PREDICTED: peptidyl-prolyl cis-trans isomerase A-like [Mus musculus]‡ | 2154 | 59 | –1.6 | .01 |
| PREDICTED: peptidyl-prolyl cis-trans isomerase A-like [Mus musculus]‡ | 2182 | 60 | –1.4 | .01 |
| Destrin [Mus musculus]‡ | 2118 | 61 | –1.5 | .07 |
| Destrin [Mus musculus]‡ | 2129 | 62 | –3.1 | .03 |
| Peroxisomal membrane protein 20 [Mus musculus] | 2204 | 63 | –2.1 | .01 |
| Myosin light polypeptide 6 [Mus musculus] | 2239 | 64 | –1.5 | .001 |
* Randomly selected tumor specimens from three mice in each group were used for proteomics analysis.
† All P values were calculated by a two-sided Student t test.
‡ The protein spots likely represent isoforms or posttranslational modification(s) of the same protein.