Figure 3.

Protein domain structure and phylogenetic analysis of Tudor domain-containing 7 sequences. (A) Schematic depiction of the composition of major domains in B. floridae Tudor domain-containing 7 (Tdrd7) protein (predicted from clone bfga025n02) and related proteins from Drosophila melanogaster (Dm) and human (Hs). Conserved protein domain information is provided in Additional file 2: Table S2. (B) Phylogenetic tree of B. floridae Tdrd7 and other related proteins constructed using the neighbor-joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to each branch. When the supporting percentage of a particular node is less than 60, the value is not shown on the node. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the p-distance method and are presented as the number of amino acid differences per site. The analysis involved 16 amino acid sequences, and their NCBI accession numbers are listed in Additional file 3. Species abbreviations: Bf, Branchiostoma floridae (Florida amphioxus); Dm, Drosophila melanogaster (fruit fly); Dr, Danio rerio (zebrafish); Hs, Homo sapiens (human); Mm, Mus musculus (mouse); Xl, Xenopus laevis (African clawed frog).