Figure 4. Time-course methylation and expression analysis for HSPA2, TNF, and TYROBP in AZA treated SKM-1 cells.

Top, CG methylation as determined by pyrosequencing. Y-axes indicate mean percentage of methylation at 3 (HSPA2), 5 (TNF), and 4 CpG sites (TYROBP). Bottom, qRT-PCR data showing relative gene expression normalized to the housekeeping gene GAPDH. No drug was applied to the cells from day 28 to day 38. Both expression and methylation data are means (± SEM) from four independent experiments obtained from mock- (green line) and AZA- (red dashed line) treated cells.