Figure 2. MSC pretreatment attenuated kidney inflammation in I/R-induced AKI.

The MSCs (1 × 10⁶) were intraperitoneally administered 4 h before I/R and the kidney inflammation was assessed by immunohistochemical detection of neutrophils and macrophages, and kidney cytokine concentration. (A) Neutrophil (Ly6G) immunohistochemistry, magnification ×200. (B) Macrophage (F4/80) immunohistochemistry, magnification ×200. (C) The kidney cytokine concentration was measured by CBA (n = 5–6 per group). * p < 0.05 compared to the sham, ** p < 0.05 compared to PBS + I/R, # p < 0.05 compared to MSC + I/R.