Figure 7. The beneficial effect of the MSCs was completely abrogated in CD11c⁺ cell-depleted mice.

For CD11c⁺ cells depletion, DT (4 ng/g) was intraperitoneally administered to CD11c-DTR transgenic mice and WT mice. At 18 h later, flow cytometric analysis of the spleen and kidney CD11c⁺ cells was performed. (A) Depletion of spleen CD11c^high CD11b⁺ DCs and kidney CD11c⁺ MHC II⁺ cells was confirmed. (B) Serum creatinine, (C) the tubular injury score, (D) the mean numbers of apoptotic cells per HPF and (E) FoxP3 mRNA expression in kidney were measured at 24 h after renal I/R in MSC- or PBS-pretreated mice (WT and CD11c-DTR transgenic mice). n = 6–7 per group, * p < 0.05 compared to PBS + I/R.