Table 1. Restriction sites and sequences of primers used for cloning.
Gene | Forward | Reverse | ||
Restriction Site | Sequence | Restriction Site | Sequence | |
pOX-hENT1 | SalI | 5′-GCACGTCGACCAATAATGACAACCAGTCAC-3′ | XbaI | 5′-CGTGTTCTAGATCACACAATTGCCCG-3′ |
pOX-hENT2 | SalI | 5′-GATATAGTCGACCAATAATGGCGCGAGGAGAC-3′ | XbaI | 5′-CTATCTCTAGATCAGAGCAGCGCCTTGAAGAG-3′ |
pOX-hCNT2 | HindIII | 5′-GCCCGAAGCTTCAATAATGGAGAAAGCAAGTGG-3′ | XbaI | 5′-CGTCGTCTAGATTAGGCACAGACGGTATTG-3′ |
pOX-hCNT3 | HindIII | 5′-CGCACAAGCTTCAATAATGGAGCTGAGGAG-3′ | SpeI | 5′-CCGCGCACTAGTTCAAAATGTATTAGAGATCCC-3′ |