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. 2013 Aug 6;8(8):e71196. doi: 10.1371/journal.pone.0071196

Table 1. Restriction sites and sequences of primers used for cloning.

Gene Forward Reverse
Restriction Site Sequence Restriction Site Sequence
pOX-hENT1 SalI 5′-GCACGTCGACCAATAATGACAACCAGTCAC-3′ XbaI 5′-CGTGTTCTAGATCACACAATTGCCCG-3′
pOX-hENT2 SalI 5′-GATATAGTCGACCAATAATGGCGCGAGGAGAC-3′ XbaI 5′-CTATCTCTAGATCAGAGCAGCGCCTTGAAGAG-3′
pOX-hCNT2 HindIII 5′-GCCCGAAGCTTCAATAATGGAGAAAGCAAGTGG-3′ XbaI 5′-CGTCGTCTAGATTAGGCACAGACGGTATTG-3′
pOX-hCNT3 HindIII 5′-CGCACAAGCTTCAATAATGGAGCTGAGGAG-3′ SpeI 5′-CCGCGCACTAGTTCAAAATGTATTAGAGATCCC-3′