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. 2013 Aug 6;8(8):e72404. doi: 10.1371/journal.pone.0072404

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© 2013 Wu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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RAW 264.7 were pretreated with TLR4 antibody (AB) for 30 min, and then treated with LPS. Levels of nuclear GATA-2 were immunodetected (A, top panel). PCNA was determined as the internal controls (bottom panel). These immunorelated protein bands were quantified and statistically analyzed (B). RAW 264.7 cells were transfected to TLR-4 small interference (si) RNA for 24 and 48 h. Levels of TLR-4 were immunodetected (C, top panel). β-Actin was measured as the internal control (bottom panel). These protein bands were quantified and statistically analyzed (D). RAW 264.7 cells were exposed to LPS, TLR4 siRNA, and a combination of TLR4 siRNA and LPS. Amounts of GATA-2 were immunodetected (E, top panel). PCNA was measured as the internal control (bottom panel). These protein bands were quantified and statistically analyzed (F). The immunoblotting results shown are a representative of 6 experiments, and the other statistically analyzed results are a compilation of 6 replications. Each value represents the mean ± SD. An asterisk (*) and pound sign (^#) respectively indicate that the value significantly (p < .05) differed from the respective control and LPS-treated group.