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. 2013 Aug 6;8(8):e72270. doi: 10.1371/journal.pone.0072270

Figure 1. Splicing of BARE transcripts.

Figure 1

A. Agarose gel electrophoresis of the RT-PCR amplification product from callus RNA using primers LS1 and AP4. The upper band (arrow, 1.3 kb) contains products from both BARE1 and BARE2 and is the same size as the amplification product from genomic DNA using the same primer pair (B); the lower, faint band (A, arrow, 1.2 kb) is the spliced BARE1 form and it is not seen in the genomic DNA amplification. B. RT-PCR (+) from total RNA (primers LS1 and AP4); the lanes display reactions containing reverse transcriptase (+), negative control lacking reverse transcriptase (-), or genomic DNA instead of RNA (G) as the positive control. C. Detection of splicing in embryo, E, and callus, C, total and poly(A) RNA (labelled p (A)) using a BARE1-specific primer pair (81567, F1594). Arrows indicate the unspliced and spliced forms. Size markers (m), 100 bp ladder, marked band is 1 kb (A, B) or 0.5 kb (C).