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. 2013 Aug 6;8(8):e68576. doi: 10.1371/journal.pone.0068576

Figure 4. Strand-displacement activity of DNA polymerases beta (A, B) and lambda (C, D) in the presence of Mg2+ (A, C) or Mn2+ (B, D) ions using DNA1 (lanes 1–6), DNA2 (lanes 7–12), DNA3 (lanes 13–18) and DNA4 (lanes 19–24).

Figure 4

Lane C, position of the 5'–(32P)labeled 55 and 18 nt-long DNA; lanes 1, 7, 13 and 19 (upper panel), initial DNA substrate after UDG treatment; lanes 2, 8, 14 and 20 (upper panel), and 1, 7, 13 and 19 (bottom panel), initial DNA substrate after sequential treatment by UDG and hAPE1; 18, 19 and 20 nt, lengths of the reaction products. The final concentrations of dNTPs are indicated below the gels. The autoradiographs present the results from one of three independent experiments.