Table 1. DNA substrate constructs used in the present study.
name | sequences | primer length (nts)a |
DNA1 | 5'-GACTACATTTCATCTGGCTTGGGCTTCATCGTTGTCdUfCAGACCTGGTGGATACCG | 17 |
3'-CTGATGTAAAGTAGACCGAACCCGAAGTAGCAACAG A OTCTGGACCACCTATGGC* | ||
DNA2 | 5'-GACTACATTTCATCTGGCTTGGGCTTCATCGTTGTCdUfCAGACCTGGTGGATACCG | 18 |
3'-CTGATGTAAAGTAGACCGAACCCGAAGTAGCAACAG O GTCTGGACCACCTATGGC* | ||
DNA3 | 5'-GACTACATTTCATCTGGCTTGGGCTTCATCGTTGTCdUfCAGACCTGGTGGATACCG | 19 |
3'-CTGATGTAAAGTAGACCGAACCCGAAGTAGCAACAO A GTCTGGACCACCTATGGC* | ||
DNA4 | 5'-GACTACATTTCATCTGGCTTGGGCTTCATCGTTGTCTCAGACCTGGTGGATACCG | 18 |
3'-CTGATGTAAAGTAGACCGAACCCGAAGTAGCAACAGOGTCTGGACCACCTATGGC* |
primer length – the length of the primed oligonucleotide created under hAPE1 activity, dUf –5-formyl-2'-deoxyuridine, O – AP site, * –32P-radioactive label.