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. 2013 Aug 6;8(8):e68576. doi: 10.1371/journal.pone.0068576

Table 1. DNA substrate constructs used in the present study.

name sequences primer length (nts)a
DNA1 5'-GACTACATTTCATCTGGCTTGGGCTTCATCGTTGTCdUfCAGACCTGGTGGATACCG 17
3'-CTGATGTAAAGTAGACCGAACCCGAAGTAGCAACAG A OTCTGGACCACCTATGGC*
DNA2 5'-GACTACATTTCATCTGGCTTGGGCTTCATCGTTGTCdUfCAGACCTGGTGGATACCG 18
3'-CTGATGTAAAGTAGACCGAACCCGAAGTAGCAACAG O GTCTGGACCACCTATGGC*
DNA3 5'-GACTACATTTCATCTGGCTTGGGCTTCATCGTTGTCdUfCAGACCTGGTGGATACCG 19
3'-CTGATGTAAAGTAGACCGAACCCGAAGTAGCAACAO A GTCTGGACCACCTATGGC*
DNA4 5'-GACTACATTTCATCTGGCTTGGGCTTCATCGTTGTCTCAGACCTGGTGGATACCG 18
3'-CTGATGTAAAGTAGACCGAACCCGAAGTAGCAACAGOGTCTGGACCACCTATGGC*
a

primer length – the length of the primed oligonucleotide created under hAPE1 activity, dUf –5-formyl-2'-deoxyuridine, O – AP site, *32P-radioactive label.