Table 3. Efficiency (kcat/Km, μM−1⋅sec−1) of dNMPs incorporation by DNA polymerases beta and lambda via the long-patch BER pathway.
pol λ | pol β | |||
Mg2+ | Mn2+ | Mg2+ | Mn2+ | |
DNA1 | 0.003 | 0.0084 | 0.0084 | 0.012 |
DNA2 | 0.01 | 0.0003 | 0.0034 | 0.037 |
DNA3 | 0.022 | 0.0164 | 0.0131 | 0.235 |
DNA4 | 0.008 | 0.0072 | 0.0074 | 0.118 |
Note: the results are presented as the average value of three independent experiments. Standard error was estimated as 10%.