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. 2013 Aug 6;8(8):e71886. doi: 10.1371/journal.pone.0071886

Figure 4. The effect of overexpression of wild type, GTP-locked and GDP-locked Rab41 on Golgi ribbon structure.

Figure 4

HeLa cells stably expressing GalNAcT2-GFP were microinjected with pCMUIV plasmids encoding myc-tagged wild type, GTP-locked and GDP-locked Rab41, respectively. The stock plasmid concentration was 100 ng/µl. After a 36-h expression period, cells were fixed and stained with anti-myc antibody (A–C). Golgi structure was displayed by expression of Golgi-associated protein, GalNAcT2-GFP (A’–C’). In cells overexpressing GDP-locked Rab41, the Golgi apparatus was fragmented (A and A’), whereas the compact Golgi ribbon was observed in cells injected by either GTP-locked (B and B’) or wild type Rab41 (C and C’). Asterisks mark injected cells. (D) Quantitative analysis of cells overexpressing GDP-locked Rab41 displayed fragmented Golgi apparatus at 100 ng/µl plasmid concentration and ranged expression time from 8 to 36 h. ~50 injected cells were assayed for each time point.