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. 2013 Aug 6;8(8):e71886. doi: 10.1371/journal.pone.0071886

Figure 10. The effect of Rab41 knockdown or overexpression on HeLa cell growth.

Figure 10

In knockdown experiments, ~70,000 HeLa cells stably expressing GalNAcT2-GFP were transfected with either control siRNA or siRab41(4) at a concentration of 200 nM. After a 2-day delay, the siRab41(4) treated cultures showed little further increase in cell number (A). As cell number shown on the Y-axis in A is the mean of the 6 image fields sample, the total number of cells scored at each time point is 6 times the Y axis number. In microinjection experiments, HeLa cells stably expressing GalNAcT2-GFP were microinjected with a range of concentrations (50, 100 and 200 ng/µl) of pCMUIV plasmids encoding myc-tagged wild type, GTP-locked or GDP-locked Rab41. After a period of expression at 37° C, cells were fixed and stained with anti-myc antibody. (B) Number of cells overexpressing GDP-locked Rab41 at various times following plasmid injection. A constant number of cells were injected in all cases. (C) Quantitative analysis of cell survival for HeLa cells microinjected with wild type, GTP-locked or GDP-locked Rab41 following 8-h or 36-h plasmid expression. Error bars represent the mean ± range of two independent experiments.