Figure 11. Co-depletion of Rab6 and Rab41 causes Golgi fragmentation, a result similar with Rab41 knockdown alone.

HeLa cells stably expressing GalNAcT2-GFP were transfected with control, Rab6, Rab41 siRNA or both Rab6 and Rab41 siRNAs at an individual concentration of 200 nM. Cells were fixed 96 h post initial transfection. Rab6 knockdown alone had no effect on Golgi ribbon structure (B). However, when Rab6 and Rab41 were co-depleted, the Golgi apparatus was fragmented (D). This Golgi phenotype is similar with that of Rab41 knockdown alone (C). (E) Quantitative analysis of cells displayed fragmented Golgi. Error bars represent the mean ± SEM of three replicates. ~100 cells were assayed for each condition.