Table 1. Quantification of Golgi morphology in siControl and siRab41(4) transfected cells.
Experiment 1
| |||||
---|---|---|---|---|---|
siControl Golgi | |||||
Cell | Number of stacks per Golgi rich area | Average number of Golgi-associated vesicles per stack | Average maximum cisternae length (nm) | Average Golgi stack width (nm) | Average number of cisternae per stack |
1 | 4 | 5 | 1022 | 240 | 4 |
2 | 3 | 10 | 1287 | 403 | 4 |
3 | 3 | 7 | 747 | 198 | 3 |
4 | 5 | 8 | 912 | 296 | 3 |
5 | 3 | 7 | 1084 | 325 | 4 |
Average ± SEM | 3.6 ± 0.4 | 7.4 ± 0.8 | 1010.4 ± 89.8 | 292.4 ± 35.3 | 3.6 ± 0.2 |
siRab41(4) Golgi | |||||
1 | 6 | 13 | 405 | 254 | 3 |
2 | 4 | 14 | 380 | 243 | 2 |
3 | 4 | 13 | 356 | 342 | 3 |
4 | 6 | 12 | 426 | 375 | 2 |
5 | 5 | 13 | 356 | 287 | 3 |
6 | 3 | 17 | 473 | 285 | 3.00 |
Average ± SEM | 4.7 ± 0.5 | 13.7 ± 0.7 | 399.3 ± 18.5 | 297.7 ± 20.9 | 2.7 ± 0.2 |
Experiment 2 | |||||
siControl Golgi | |||||
1 | 4 | 9 | 865 | 312 | 3 |
2 | 2 | 5 | 803 | 240 | 5 |
3 | 2 | 9 | 1078 | 334 | 4 |
4 | 4 | 8 | 777 | 301 | 4 |
5 | 3 | 7 | 841 | 321 | 4 |
6 | 5 | 9 | 770 | 310 | 3 |
Average ± SEM | 3.3 ± 0.5 | 7.8 ± 0.7 | 855.7 ± 46.9 | 303.0 ± 13.4 | 3.8 ± 0.3 |
siRab41(4) Golgi | |||||
1 | 5 | 18 | 500 | 314 | 3 |
2 | 5 | 9 | 384 | 290 | 2 |
3 | 8 | 15 | 501 | 255 | 3 |
4 | 6 | 11 | 399 | 309 | 3 |
5 | 4 | 15 | 403 | 223 | 2 |
6 | 9 | 11 | 464 | 298 | 2 |
7 | 7 | 12 | 485 | 322 | 3 |
8 | 5 | 14 | 452 | 286 | 2 |
Average ± SEM | 6.1 ± 0.6 | 13.1 ± 1.0 | 448.5 ± 16.7 | 287.1 ± 11.7 | 2.5 ± 0.19 |
Overall Average | |||||
siControl Golgi | 3.5 | 7.6 | 933.0 | 297.7 | 3.7 |
siRab41(4) Golgi | 5.4 | 13.4 | 423.9 | 292.4 | 2.6 |