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. 2013 Aug 6;8(8):e70565. doi: 10.1371/journal.pone.0070565

Figure 2. Curcumin, NAC, TEA and 4-AP alleviated HIV-1 gp120 V3 loop-induced ROS increase in microglia.

Figure 2

After pre-incubation of microglia with curcumin (15 µM), NAC (3 mM), TEA (2 mM) or 4-AP (2 mM) for 2 h, cells were treated with HIV-1 gp120 V3 loop (1 µg/mL) for 1 h, and then subjected to ROS measurement using fluorescence microscope and FACS. A) and B): ROS production in microglia was determined using fluorescence microscope. The arrows indicated ROS-positive microglial cells. C) and D): ROS production in microglia was determined using FACS. Six independent experiments were performed. Each bar graph shows changes of ROS MFI calculating from comparison of experimental group versus medium control. *, P<0.05 vs. control, n = 6; #, P<0.05 vs. HIV-1 gp120 V3 loop alone, n = 6. Cur, curcumin.