Skip to main content
. 2013 Aug 6;8(8):e70565. doi: 10.1371/journal.pone.0070565

Figure 3. Curcumin, NAC, 4-AP and TEA reduced HIV-1 gp120 V3 loop-induced TNF-α and MCP-1 mRNA expression in microglia.

Figure 3

After pre-incubation of microglia with curcumin (15 µM), NAC (3 mM), TEA (2 mM) or 4-AP (2 mM) for 2 h, cells were treated with HIV-1 gp120 V3 loop (1 µg/mL) for 3 h. Cells were then subjected to RNA extraction for measuring TNF-α and MCP-1 mRNA expression using a qPCR assay. A) and B): TNF-α mRNA and MCP-1 mRNA expression, respectively. *, p<0.05 vs. control, n = 6; #, p<0.05 vs. HIV-1 gp120 V3 loop alone. Experiments were repeated 6 times (n = 6). Cur, curcumin.