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. 2013 Aug 6;8(8):e70398. doi: 10.1371/journal.pone.0070398

Figure 4. RXR-γ levels sensitize cellular apoptosis in A4-T cells upon retinoid treatment.

Figure 4

A. Annexin V-FITC assay data showing apoptosis in A4-P and A4-T cells on different retinoids treatment regimes; where i. having no retinoid treatment, ii. treated with CRA, iii. with ADA and iv. with both having alternative treatment of another synthetic retinoid i.e. TTNPB. B. Statistical analysis of apoptosis assay showing significant apoptosis among both cell types in different sets of retinoid treatment. C. Expression analyses of RXR-γ, PPAR-γ, Bcl-2, Caspase 9 and snail at transcriptional (Tr.) and protein level (Pr.) through RT-PCR and immunoblotting respectively. D. Quantitation of mRNA expression, i. expression of RXR-γ, PPAR-γ, Caspase 9, Bcl-2 and snail upon retinoids treatment in A4-P cells whereas, ii. shows their levels in A4-T cells on validation through RT-PCR. E. Quantitation of protein expression, i. expressions of RXR-γ, PPAR-γ, Caspase 9, Bcl-2 and snail in A4-P cells whereas, ii. shows their levels in A4-T cells upon retinoids treatment validated through immunoblotting.