Table 1. Summary of the structural analysis of various mutants of human γD crystallin.

S.No.	Mutants	Secondary structure and solvent accessibility of the residue	Non polar residues with increased solvent exposure in the mutant*	Polar residues with increased solvent exposure in the mutant*	Effect on Inter-domain interactions	Intermolecular interactions at the site of mutation
1	P24T	Edge strand in the second Greek key (GK) motif in the N-terminal domain is solvent-exposed	Y6,Y16,Y45,Y50,Y62, A63,Y98,F118,W157,A159	T4,E7,R9,Q12,R14,H15,E17,D21,P23),S30,N33,R36,D38,Y45,Q47,S51,R59,D61,H65Q66,Q67,S72,D73,R76,S87,H88,R89,R95,R99,Q101,C111,Q113,R115,N125,E128,R140,T160,R163	Indirectly	Not directly affected
	A36P	GK-1motif disturb due to Proline	P43,L53, F118,F173	R9,N33, C41,Q47, H65,Q66, D 97,R99,C111,	GK1 not involved in interdomain interactions. However, the conformation change and destabilization of GK1 might indirectly affect.	Altered stability and conformation of the GK1 might affect Intermolecular interactions
3	L45PL54P	Residues are in the middle and edge strands, respectively, in GK 2. L45 is buried, L54 is solvent-exposed	L53, M69, L71,	R9, E46, D64, Q66, Q67, S74, R76, R99	GK2 involved in domain-domain interactions. The conformation and stability of this β sheet is affected due to the presence of prolines	Altered stability and conformation of the GK2 might affect Intermolecular interactions
4	R77S	One of the middle strands in GK motif 2 in N-terminal domain is solvent -exposed	Y55, F118, I171	R9, F24, Q26, N33, E46,Q47,N49,Q66,Q67,S72,S74,S77,D97,R99,Q101	No	Occurs close to a positively charged patch in symmetry- related molecule
5	E107A	Loop connecting the two GKs in C-terminal domain, solvent-exposed	L53,F105,F118	R9,N33,Q66,R76,H88,R89, D97,R99,Q101,T106,D108,C109,R169	No	Polar environment in the symmetry- related molecule
6	Y134A	Located at the middle strand in GK4, buried	L53, M69, F118, V132, Y139,	R9,N33, E46, D64, N66, N67, S74, R76, D97, R99, Q101,R140, Q143	Possibly affected as the site occurs in GK4 involved in inter-domain interactions	Does not seem to be affected
7	R140X	Three strands corresponding to both the motifs and a loop connecting the two strands	L53,F56,I81,I90,L92,Y98,L112,I121,L124,V126,L127,W131,V132,L133,Y134,L136,	Y45,Q54,R59,R79,S84,H88,E96,D97,Y98,T106,D108,C109,Q113,N119,E120,H122,N125,S130,E135,S137,N138, #Y144,L145,L146,W157,A159,A162,V164,L167,V170	Affected	Could be affected due to unfolding of the molecule
8	W157X	Loop and beta strand at the C-terminal region	F56,I81,L92,L112,I121,L124,W131,V132,L133,Y134,L136,Y139,Y144,L146,Y151,Y154,	Q54,R59,S84,H88,T106,D108,C109,Q113,N119,E120,S130,E135,S137,R142,Y144,Y154, #W157,A159,A162,V164,L167,V170	Affected	Could be affected due to unfolding of the molecule
9	G165fs		L53,F56,I81,L92,F118,W131,V132,L133,Y139,	R9,N33,R59,Q66,Q67,R76,S84,H88,D97,R99,Q101,T106,D108,C109,S130,Y134,#V170	Affected	Possibly affected due to unfolding

^#The residues indicated in italics are the residues buried in the WT but absent in the respective mutants.